Detection of pulmonary tuberculosis using cartridge based nucleic acid amplification test (CBNAAT) and fluorescent microscopy

AUTHOR(s) : Naorem Salinita1, Laifangbam Supriya2, Mutum Usharani3, Huidrom Lokhendro Singh4
DOI No. :

Background: With the advent of advanced laboratory methods for diagnosis of Pulmonary Tuberculosis (PTB), its detection now is increasingly relied upon rapid diagnostic methods like Cartridge based nucleic acid amplification test (CBNAAT) and Fluorescent Microscopy (FM). No study has been published regarding the effectiveness of CBNAAT and FM. This study aims to better the understanding of these methods for detection of PTB. The objective of the study was to compare CBNAAT and FM for detection of Mycobacterium tuberculosis (MTB) using sputum samples. Methods: A cross-sectional study was performed among 200 study population. 3 sputum samples from each patient were subjected to CBNAAT and 2 slide smears were prepared for Aura mine- O stained FM. Statistical analysis was done using SPSS 23 by Chi square test. P value less than 0.05 was taken as significant difference for this study.

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