A study on isolation and identification of bacterial agents responsible for postoperative wound infection

AUTHOR(s) : Sarma MC, Das DK
DOI No. : 10.31741/ijhrmlp.v5.i2.2019.4

ABSTRACT :
The term post-operative wound infection, also known by the term surgical site infection (SSI) is as old as the beginning of surgery. The majority of post-operative wound infection (SSI) become apparent within 30 days of an operative procedure and most often between 5th and 10th postoperative days. Materials and methods: This was a hospital-based observational, descriptive study carried out on 2685 SSI wound samples were included in the present study collected from General Surgery, Orthopedic, Obstetrics, and Gynaecology Departments. Results: In the present study in the clean wound category with no obvious source of contamination, 65.8% of the cultured infected wounds were of monomicrobial etiology. The isolates when compared with the duration of surgery, it was found that with longer durations of surgery, the wound was infected with polymicrobial agents, The incidence of Klebsiella, E.coli, and Pseudomonas increased with longer duration of surgery. This suggests that the organisms might be transferred to the wound by prolonged contact with the operating staff and equipment, as the airborne spread of the Gram-negative organisms is rare. Conclusion: The present study has enlightened the relationship between SSI, preoperative hospitalization and duration of surgery. There was an increase in the incidence of infection, in patients with longer preoperative hospitalization and longer durations of surgery. There was an increase in poly-microbial etiological agents in these cases. Klebsiella was found to be the main etiological agent followed by E. coli, Pseudomonas, Coagulase-negative staphylococci, etc.

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